Immune-Related Genetic Overlap Between Regional Gray Matter Reductions and Psychiatric Symptoms in Adolescents, and Gene-Set Validation in a Translational Model

Frontiers in systems neuroscience

Front Syst Neurosci. 2021 Sep 30;15:725413. doi: 10.3389/fnsys.2021.725413. eCollection 2021.

ABSTRACT

Adolescence is a period of vulnerability for the maturation of gray matter (GM) and also for the onset of psychiatric disorders such as major depressive disorder (MDD), bipolar disorder and schizophrenia. Chronic neuroinflammation is considered to play a role in the etiology of these illnesses. However, the involvement of neuroinflammation in the observed link between regional GM volume reductions and psychiatric symptoms is not established yet. Here, we investigated a possible common immune-related genetic link between these two phenomena in european adolescents recruited from the community. Hippocampal and medial prefrontal cortex (mPFC) were defined a priori as regions of interest (ROIs). Their GM volumes were extracted in 1,563 14-year-olds from the IMAGEN database. We found a set of 26 SNPs that correlated with the hippocampal volumes and 29 with the mPFC volumes at age 14. We formed two ROI-Related Immune-gene scores (RRI) with the inflammation SNPs that correlated to hippocampal GM volume and to mPFC GM volume. The predictive ability of both RRIs with regards to the presence of psychiatric symptoms at age 18 was investigated by correlating the RRIs with psychometric questionnaires obtained at age 18. The RRIs (but not control scores constructed with random SNPs) correlated with the presence of depressive symptoms, positive psychotic symptoms, and externalizing symptoms in later adolescence. In addition, the effect of childhood maltreatment, one of the major environmental risk factors for depression and other mental disorders, interacted with the RRI effect. We next sought to validate this finding by investigating our set of inflammatory genes in a translational animal model of early life adversity. Mice were subjected to a protocol of maternal separation at an early post-natal age. We evaluated depressive behaviors in separated and non-separated mice at adolescence and their correlations with the concomitant expression of our genes in whole blood samples. We show that in mice, early life adversity affected the expression of our set of genes in peripheral blood, and that levels of expression correlated with symptoms of negative affect in adolescence. Overall, our translational findings in adolescent mice and humans provide a novel validated gene-set of immune-related genes for further research in the early stages of mood disorders.

PMID:34658802 | PMC:PMC8514661 | DOI:10.3389/fnsys.2021.725413

Authors

Lukas Penninck
El Chérif Ibrahim
Eric Artiges
Victor Gorgievski
Sylvane Desrivières
Severine Farley
Irina Filippi
Carlos E A de Macedo
Raoul Belzeaux
Tobias Banaschewski
Arun L W Bokde
Erin Burke Quinlan
Herta Flor
Antoine Grigis
Hugh Garavan
Penny Gowland
Andreas Heinz
Rüdiger Brühl
Frauke Nees
Dimitri Papadopoulos Orfanos
Tomáš Paus
Luise Poustka
Juliane H Fröhner
Michael N Smolka
Henrik Walter
Robert Whelan
Julien Grenier
Gunter Schumann
Marie-Laure Paillère Martinot
Eleni T Tzavara
Jean-Luc Martinot
IMAGEN Consortium